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ICSI Treatment

The First And Pioneering ICSI Center In India Since 1994.

Infertility can be caused by a variety of factors, Hence it is important to determine the best course of treatment. In cases of male infertility, Intracytoplasmic Sperm Injection (ICSI) treatment in Mumbai may be recommended. ICSI is a procedure where a single sperm is injected into an egg using very fine micromanipulation equipment. This can overcome the block to fertilisation due to the very low count, motility or morphology of the sperm. ICSI is an advanced form of IVF. The IVF treatment cycle includes ovarian stimulation and egg retrieval. It is important to consult with a fertility specialist to determine if ICSI is the right treatment for you.

The Best ICSI Treatment In India At Jaslok-FertilTree

FertilTree provides IVF and ICSI treatments in Mumbai, India for couples who are unable to conceive a child naturally. Fertiltree has helped thousands of couples fulfil their dreams of parenthood with ICSI.

Our team will work with you to determine the best course of treatment for your situation. We are committed to providing you with the highest quality care and will endeavour to fulfil your dreams.

When Do You Need An ICSI Treatment?

Low Sperm Count: If you have a low sperm count, it may be difficult to fertilize an egg naturally. ICSI can help increase the chances of fertilization by injecting a single sperm directly into the egg.

Poor Semen Quality: Another reason for ICSI is poor semen quality. Sperm with poor morphology (shape) or motility (movement) may have difficulty fertilizing an egg on their own. Injecting a single sperm into an egg bypasses the various blocks to fertilisation and allows for fertilization to occur as the sperm is injected into the body of the egg.

Previous vasectomy or a failed vasectomy reversal:  In these situations, ICSI would be the appropriate choice. The sperm would be collected from the epididymis or the testes.

The Process Of Treatment with ICSI

Ovarian Stimulation: The female partner is given medication to boost the development of follicles in the ovary starting usually on Day 2 of menses. The stimulation continues till the follicles are ready with mature eggs.

Collection of sperm and their processing: Once the sample is collected by masturbation, it is handed over to the embryology laboratory where the embryologist processes it in a gentle way to obtain viable and healthy sperm.

Egg Retrieval: The hormonal stimulation culminates with egg retrieval. The oocytes are collected under mild anaesthesia under transvaginal ultrasound guidance. 

Examination of the Eggs and the Sperm: The eggs and the sperm are examined under the microscope to determine their quality. Once that is assessed, they are prepared for the ICSI procedure.

Introduction of sperm into the egg: ICSI is carried out using a joystick, a micromanipulator system, and a microscope.  


In this procedure, the oocyte is prepared by removing its outer coat of cells called the cumulus complex. Once laid bare, the oocyte is held gently by a holding pipette under an inverted microscope. The sperm that has been prepared for microinjection is injected through a very fine micropipette into the cytoplasm (body) of the oocyte. The oocyte holding pipette and the injection pipette are 20 and 60 times thinner than human hair respectively. The egg itself is no bigger than the tip of a pin. The microinjected egg is returned to the incubator and checked the following day for fertilization.

Micromanipulation / Intra Cytoplasmic Sperm Injection – Movie Clip

Micromanipulation is ideally suited in cases of very low sperm counts (oligozoospermia) or zero counts (azoospermia), very little (asthenospermia) or complete lack of motility and abnormal shape of the sperm (teratozoospermia). Its use is extended to women of advanced age, women suffering from endometriosis and those with immunological and unexplained infertility. Preimplantation GEnetic Testing (PGT) can be done on the embryos formed after ICSI. Current thinking permits the use of ICSI for many forms of infertility.

Dr. Firuza Parikh’s team achieved the first ICSI pregnancy in South East Asia in 1994. Luv Singh is South East Asia’s first ICSI baby.

Intracytoplasmic Morphologically Selected Sperm Injection (IMSI)

This technique has added a new dimension to the procedure of ICSI. Normally the procedure of ICSI is carried out at a magnification that is twenty-fold. The IMSI procedure uses a specialized lens which magnifies the sperm 7000 times. This makes the isolation of normal-looking sperm easier. IMSI is particularly useful when there is severe male factor infertility, with a large proportion of abnormal-looking sperm, very compromised sperm motility and count and repeatedly failed IVF procedures.

Culture of the embryos: After fertilisation, the embryos are cultured in the warmth of the incubator for 3 to 5 days. 

Embryo transfer into the uterus: Once the embryos are ready, usually a single good-quality embryo is transferred into the uterus where it will implant.

How Much Does ICSI Treatment In India Cost?

The cost of an ICSI procedure depends on where you are located. Some factors that can influence the cost include the number of cycles of ICSI required, the cost of medications, monitoring tests, whether PESA and TESA are required and other associated expenses.

At Jaslok-FertilTree, we strive to provide each patient with the best possible experience and service. Please call us to set up an appointment and learn more about our ICSI treatment.


Intra-cytoplasmic sperm injection (ICSI): It involves injecting a single sperm directly into the egg. It is an advanced form of in vitro fertilization (IVF).

ICSI is used when:

  • Sperm have poor count, motility, or morphology (structure), or they are absent in the semen due to obstructive or non-obstructive azoospermia.
  • In the presence of  unexplained fertility problems 
  • When other assisted reproductive technologies (ART), such as IVF, have failed.  
  • When there is a diminished reserve of eggs

IVF is a process in which 50,000 processed sperm are added to the dish containing the oocyte. ICSI is an advanced form of IVF in which a single sperm is injected into the egg. This increases the chances of the egg getting fertilized.

IVF was introduced by Edwards and Steptoe in the late 1970s whereas  ICSI was developed by Palermo in 1992. The first ICSI baby in India was created by our team in 1994. Both procedures are safe and effective, but ICSI may be preferable in some situations.

The ICSI Procedure does not always result in twin pregnancy. The chances of twins increase when 2 or more embryos are transferred into the uterus. This is applicable to IVF and ICSI.

ICSI mimics nature very closely and performs the 7 steps of fertilisation.